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Best way to pipet 0.1 mlExpand / Collapse
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Posted 7/2/2008 6:46:34 PM
Supreme Being

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Our venerable 100 ul Oxford fixed volume (over 22 years old) is ready for the museum.  We use Rainin and Eppendorf pipettors in other parts of the lab, but not for volumes as small as 0.1 ml.

Since we decided to use 0.1 ml volumes for our spikes we'd like to get a new 100 ul pipettor.

So...we'd like to know which models/brands you prefer.

Post #9542
Posted 7/6/2008 12:17:18 AM
Supreme Being

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I have experienced with Eppendorf, Brand and Gilson brands. I found these all are very good for long time smooth operations.

sabir
Post #9563
Posted 7/7/2008 6:04:24 PM
Supreme Being

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The brand I like the best is Eppendorf. They have adjustable ones that will let you pipette anywhere from 10-100 uL.

Christopher Fair
R&D Chemist III
Hach Company
cfair@hach.com
www.boddoctor.com
Post #9580
Posted 7/8/2008 5:55:26 AM
Supreme Being

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If you will be measuring 0.1ml most of the time I will recommend to purchase a fixed volume micropipette. This will ensure to reduce the chances for unnoticed variations especially during single sitting sloppy operations or alternatively if you need  a variable volume micropipette then a brand with lock system as in Gilson is a better choice.

sabir
Post #9582
Posted 7/17/2008 8:07:33 PM
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We use the Rainin EDP Plus pipets, for the most part, and they work very well for us.  (I regularly use one to pipet 40 uL and 70 uL for my two low ortho-PO4 calibrants!)  We also have a Rainin EDP3 that works fine (though unlike the EDP Plus, you have to use arrow keys and scroll to set your volumes).  Both these models are motorized.  We've been using Rainins for our small-volume needs for over 10 years now.  They last a long time in Nutrients & BOD (we may even still have some of the original ones in those departments).  In Metals, their lifetime is noticeably shorter.  I think the acid fumes may corrode them.  I'll add that Rainin has a competent service department that re-calibrates pipets and does repairs.  They'll also provide you a certificate of calibration.
Post #9684
Posted 7/23/2008 1:40:26 PM
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We have in our lab Eppendorf, Rainin, and Hach 10-set pipettes- I am the official pipette calibrator for all of them. My preference is for the Eppendorf- they are very easy to calibrate and maintain. The Rainin bothers me because once the calibration goes, there's nothing you can do about it but send it back to the manufacturer. We have also noticed that the calibration will start to falter when your battery starts to wear out. You have no way of knowing that unless you are constantly checking the volume. The Hach 10-set pipettes work amazingly well, believe it or not. They are very easy to use and maintain, but a bit of a pain to calibrate. And it is super easy to order replacement parts.

--Kristina
Post #9739
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