Water Environment Federation (WEF) Discussion ForumsWater Environment Federation (WEF) Discussion Forumshttp://www.wef.org/TechnicalDiscussions/jfuller@wef.orgMon, 08 Sep 2008 02:55:19 GMT20http://www.wef.org/TechnicalDiscussions/Topic9058-14-1.aspxIf you are using Polyseed I do believe that their instrucitons specifically say to not filter the rehydrated seed solution. All that you can do is let it settle out before pipetting out your aliquots. Good luck! <img align="absmiddle" src="http://www.wef.org/TechnicalDiscussions/Skins/WEFTest2/Images/EmotIcons/Smile.gif" border="0" title="Smile"></P><P>EgadzFri, 09 May 2008 14:08:39 GMTEgadzhttp://www.wef.org/TechnicalDiscussions/Topic9058-14-1.aspxFiltering is problematic. The TSS filter would take out most of your bugs. So what size filter would work? We use the Polyseed, Put it in an erlenmeyer flask with diltuion water. Aerate for one hour. Allow to settle for 30 minutes, and then carefully decant into a beaker. The shape of the erlemeyer helps to maximize removal of the bran.</P><P>YMMV (Your Milleage May Vary)</P><P>MLAFri, 09 May 2008 11:22:19 GMTMoscowLabAnalysthttp://www.wef.org/TechnicalDiscussions/Topic9058-14-1.aspxI'm assuming you're using the commercial seed (Polyseed or Biosystems)? If so, I just let the particles settle for about 15 minutes or so and transfer the clear portion to a clean beaker.</P><P>I would think that filtering the seed would possibly trap some of the bacteria along with the particles.Tue, 06 May 2008 14:00:55 GMTSueKruegerhttp://www.wef.org/TechnicalDiscussions/Topic9058-14-1.aspxIs it possible to filter BOD seed to remove the particulate matter?Mon, 05 May 2008 13:26:28 GMTweffster